Human BACH1 ELISA Kit (Colorimetric)

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ELISA: Human BACH1 ELISA Kit (Colorimetric) [NBP3-18220] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and cell culture supernates
Standard Curve Range
46.87-3000 pg/ml
Sensitivity
22.15 pg/ml

Order Details

Human BACH1 ELISA Kit (Colorimetric) Summary

Description
The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human BACH1 in the sample, this Human BACH1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human BACH1 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Standard Curve Range
46.87-3000 pg/ml
Sensitivity
22.15 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 10
Spike Recovery
81-113%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
BACH1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Balance Solution
  2. Enzyme Conjugate
  3. Microtiter Plate
  4. Standards
  5. Stop Solution
  6. Substrate A
  7. Substrate B
  8. Wash Solution

Alternate Names for Human BACH1 ELISA Kit (Colorimetric)

  • BACH1
  • basic region leucine zipper transcriptional regulator BACH1
  • BTB and CNC homolog 1
  • BTB and CNC homology 1, basic leucine zipper transcription factor 1
  • BTB
  • HA2303
  • transcription regulator protein BACH1
  • transcription regulator protein10BACH-1

Background

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol BACH1