B7-H1/PD-L1/CD274 Antibody


Western Blot: B7-H1/PD-L1/CD274 Antibody [NBP2-15791] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: CD274 Antibody [NBP2-15791] - Immunohistochemical analysis of paraffin-embedded colon, using antibody at 1:100 dilution.
Western Blot: B7-H1/PD-L1/CD274 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD274 antibody diluted by 1:500.
Western Blot: B7-H1/PD-L1/CD274 Antibody [NBP2-15791] - A549 whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:500.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
3.47 mg/ml

Order Details

B7-H1/PD-L1/CD274 Antibody Summary

Synthetic peptide contain a sequence corresponding to a region within amino acids 230 and 290 (Q9NZQ7) of CD274
Isoform 1: Cell membrane; Single-pass type I membrane protein; Isoform 2: Endomembrane system
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
1XPBS, 20% Glycerol (pH7).
0.025% Proclin 300
3.47 mg/ml
Immunogen affinity purified


Application Notes
suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
33 kDa

Alternate Names for B7-H1/PD-L1/CD274 Antibody

  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1


Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
Species: Hu
Applications: WB, Flow, AgAct
Species: Hu, Mu
Applications: Flow, IHC, ICC

Publications for B7-H1/PD-L1/CD274 Antibody (NBP2-15791) (0)

There are no publications for B7-H1/PD-L1/CD274 Antibody (NBP2-15791).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for B7-H1/PD-L1/CD274 Antibody (NBP2-15791) (0)

There are no reviews for B7-H1/PD-L1/CD274 Antibody (NBP2-15791). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for B7-H1/PD-L1/CD274 Antibody (NBP2-15791) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional B7-H1/PD-L1/CD274 Antibody Products

Related Products by Gene

Bioinformatics Tool for B7-H1/PD-L1/CD274 Antibody (NBP2-15791)

Discover related pathways, diseases and genes to B7-H1/PD-L1/CD274 Antibody (NBP2-15791). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for B7-H1/PD-L1/CD274 Antibody (NBP2-15791)

Discover more about diseases related to B7-H1/PD-L1/CD274 Antibody (NBP2-15791).

Pathways for B7-H1/PD-L1/CD274 Antibody (NBP2-15791)

View related products by pathway.

PTMs for B7-H1/PD-L1/CD274 Antibody (NBP2-15791)

Learn more about PTMs related to B7-H1/PD-L1/CD274 Antibody (NBP2-15791).

Research Areas for B7-H1/PD-L1/CD274 Antibody (NBP2-15791)

Find related products by research area.

Blogs on B7-H1/PD-L1/CD274

There are no specific blogs for B7-H1/PD-L1/CD274, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol CD274

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-15791 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought