B7-H1/PD-L1/CD274 Antibody


Western Blot: B7-H1/PD-L1/CD274 Antibody [NB300-903] - staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: B7-H1/PD-L1/CD274 Antibody [NB300-903] - B7-H1/PD-L1/CD274 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody [NB300-903] - Staining of Human Skeletal Muscle.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
0.5 mg/ml

Order Details

B7-H1/PD-L1/CD274 Antibody Summary

Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1
This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline, pH 7.3 with 0.5% BSA.
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


  • Western Blot 0.3-1 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx 40kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). The observed molecular weight corresponds to the glycosylated forms. IHC-P: Human placenta shows membranous staining of cytotrophoblasts. Data obtained from a previous batch (from a different goat).
Read Publication using NB300-903.

Alternate Names for B7-H1/PD-L1/CD274 Antibody

  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1


This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-H1/PD-L1/CD274 Antibody (NB300-903)(1)

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Video Protocols

WB Video Protocol

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Gene Symbol CD274

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