B7-2/CD86 Antibody (PO3.1)


Flow Cytometry: B7-2/CD86 Antibody (PO3.1) [NBP1-43382] - Analysis using the PE conjugate of NBP1-43382. Surface staining of LPS-stimulated splenocytes with Rat IgG2b kappa Isotype Control PE (open histogram) and ...read more
Flow Cytometry: B7-2/CD86 Antibody (PO3.1) [NBP1-43382] - Staining of 2-day unstimulated (left) or LPS-stimulated (right) C57BL/6 splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control Purified (open histogram) or ...read more

Product Details

Reactivity MuSpecies Glossary
Applications Flow, Func
0.5 mg/ml

B7-2/CD86 Antibody (PO3.1) Summary

The immunogen for this antibody was CD86.
DC Maturation Marker
IgG2b Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
The PO3.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions and can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Alternate Names for B7-2/CD86 Antibody (PO3.1)

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86


The PO3.1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

PE Labeled NBP2-00305

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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CD86

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