B7-2/CD86 Antibody (GL1)

Images

 
Flow Cytometry: B7-2/CD86 Antibody (GL1) [NBP1-43381] - Analysis using the PE/Cy5 conjugate of NBP1-43381. Staining of 3-day LPS activated BALB/c splenocytes with 0.03 ug of PE-Cy5 Rat IgG2a Isotype Control (open ...read more
Flow Cytometry: B7-2/CD86 Antibody (GL1) [NBP1-43381] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse ...read more
Flow Cytometry: B7-2/CD86 Antibody (GL1) [NBP1-43381] - Analysis using the FITC conjugate of NBP1-43381. Staining of LPS-stimulated splenocytes with Anti-Mouse CD86 (B7-2) FITC (left) and PE (right). Appropriate isotype ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Func, IHC, IHC-Fr, IP
Clone
GL1
Clonality
Monoclonal
Host
Rat
Concentration
0.5 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

B7-2/CD86 Antibody (GL1) Summary

Immunogen
The immunogen for this antibody was CD86.
Marker
DC Maturation Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD86
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Alternate Names for B7-2/CD86 Antibody (GL1)

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B7-2/CD86
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 amp CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CD86
Entrez
Uniprot

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