B7-2/CD86 Antibody (2D10)

Images

 
Flow Cytometry: B7-2/CD86 Antibody (2D10) [NBP1-28197] - Analysis using the PE conjugate of NBP1-28197. Staining of DBA/2 mastocytoma cell line P815 and mB7.2-lg transfected P815 cells.
Flow Cytometry: B7-2/CD86 Antibody (2D10) [NBP1-28197] - Analysis using the Allophycocyanin conjugate of NBP1-28197. Staining of DBA/2 mastocytoma cell line P815 and mB7.2lg transfected P815 cells.
Flow Cytometry: B7-2/CD86 Antibody (2D10) [NBP1-28197] - Analysis using the Biotin conjugate of NBP1-28197. Staining of mastocytoma cell line P815 adn mB7.2lg transfected P815 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-28197
    • Availability
      Product Discontinued

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B7-2/CD86 Antibody (2D10) Summary

Immunogen
Mouse B cell lymphoma cell line 5C2
Marker
DC Maturation Marker
Specificity
Mouse CD86, the B7-2 costimulatory molecule and a ligand for CD28 and CD152
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD86
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50-200 molar excess
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for B7-2/CD86 Antibody (2D10)

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

CD86/B7-2 is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80/B7-1, CD86 participates in T cell costimulation via interaction with CD28 and CD152/CTLA-4. It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes and dendritic cells. Since CD86/B7-2 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. (1-9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for B7-2/CD86 Antibody (NBP1-28197)

Discover related pathways, diseases and genes to B7-2/CD86 Antibody (NBP1-28197). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-2/CD86 Antibody (NBP1-28197)

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Pathways for B7-2/CD86 Antibody (NBP1-28197)

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PTMs for B7-2/CD86 Antibody (NBP1-28197)

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Research Areas for B7-2/CD86 Antibody (NBP1-28197)

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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Bioinformatics

Gene Symbol CD86
Entrez