B7-1/CD80 Antibody (1G10) [FITC]

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Flow Cytometry: B7-1/CD80 Antibody (1G10) [FITC] [NBP1-28027] - 3 ug/10^6 cells P815 cells transfected with CD80 or non-transfected P815 control cells were stained with rat anti-mouse CD80-FITC. Large cells were then ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-28027
    • Availability
      Product Discontinued

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B7-1/CD80 Antibody (1G10) [FITC] Summary

Immunogen
Activated 5C2 cells
Specificity
Mouse CD80 (B7-1), Mr 45-47 kDa
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD80
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunoprecipitation 1:10-1:500
  • In vitro assay

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for B7-1/CD80 Antibody (1G10) [FITC]

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. (1-4) The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. (5, 6) The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. (2, 7)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28028

Additional B7-1/CD80 Products

Bioinformatics Tool for B7-1/CD80 Antibody (NBP1-28027)

Discover related pathways, diseases and genes to B7-1/CD80 Antibody (NBP1-28027). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-1/CD80 Antibody (NBP1-28027)

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PTMs for B7-1/CD80 Antibody (NBP1-28027)

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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD80
Entrez