B7-1/CD80 Antibody (1G10)

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Flow Cytometry: B7-1/CD80 Antibody (1G10) [NBP1-28026] - Analysis using the PE conjugate of NBP1-28026. Staining of DBA/2 mastocytoma cell line P815 and mB7.lg transfected P815 cells.
Flow Cytometry: B7-1/CD80 Antibody (1G10) [NBP1-28026] - Analysis using the Allophycocyanin conjugate of NBP1-28026. Staining of DBA/2 mouse mastocytoma cell line P815 and mB7.1-Ig transfected P815 cells.
Flow Cytometry: B7-1/CD80 Antibody (1G10) [NBP1-28026] - Analysis using the Biotin conjugate of NBP1-28026. Staining of DBA/2 mastocytoma cell line P815 and mb7.1-lg transfected P815 cells.
Flow Cytometry: B7-1/CD80 Antibody (1G10) [NBP1-28026] - Analysis using the FITC conjugate of NBP1-28026. Staining of 3 ug/10^6 cells P815 cells transfected with CD80 or non-transfected P815 control cells with rat ...read more
Flow Cytometry: B7-1/CD80 Antibody (1G10) [NBP1-28026] - Analysis using the FITC conjugate of NBP1-28026. Staining of Human B-cell Burkitt's lymphoma cell line Daudi 2D10 FITC.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-28026
    • Availability
      Product Discontinued

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B7-1/CD80 Antibody (1G10) Summary

Immunogen
Activated 5C2 cells
Specificity
Mouse CD80 (B7-1), Mr 45-47 kDa
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD80
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
Application Notes
Use in Western blot reported in scientific literature (PMID 9053440). Use in Immunohistochemistry reported in scientific literature (PMID 9814503)
Publications
Read Publications using NBP1-28026.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (1G10)

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. (1-4) The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. (5, 6) The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. (2, 7)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-1/CD80 Antibody (NBP1-28026)(4)

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Showing Publications 1 - 4 of 4.
Publications using NBP1-28026 Applications Species
Schubert DA et al. Self-reactive human CD4 T cell clones form unusual immunological synapses J Exp Med Jul 4 1905 [PMID: 22312112]

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB100-77811).
Van der Merwe PA et al. CD80 (B7-1) binds both CD28 and CTLA-4 with a low affinity and very fast kinetics J Exp Med (WB)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB100-77811).
WB
Battifora M. B7.1 costimulatory molecule is expressed on thyroid follicular cells in Hashimoto's thyroiditis, but not in Graves' disease J Clin Endocr Metab (IHC)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB100-77811).
IHC
Jayakumar A et al. Leishmania major infection activates NF-kappaB and interferon regulatory factors 1 and 8 in human dendritic cells Immun Jun 30 1905

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB100-77811).

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Bioinformatics Tool for B7-1/CD80 Antibody (NBP1-28026)

Discover related pathways, diseases and genes to B7-1/CD80 Antibody (NBP1-28026). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-1/CD80 Antibody (NBP1-28026)

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Research Areas for B7-1/CD80 Antibody (NBP1-28026)

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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD80
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