B220/CD45R Antibody (HIS24 (6B6))

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-64896
    • Availability
      Product Discontinued

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B220/CD45R Antibody (HIS24 (6B6)) Summary

Immunogen
Single cell suspension of rat peyers patches
Specificity
Reacts with mature and precursor B lymphocytes. Weak reaction with marginal zone B cells and a small subset of T cells and CD45R.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2M Tris/HCl (pH 7.4) and 5 - 10% fetal Bovine serum (FBS)
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for B220/CD45R Antibody (HIS24 (6B6))

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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B220/CD45R NB100-64896

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Bioinformatics

Gene Symbol PTPRC
Entrez
Uniprot