B-Raf Antibody (OTI1D2)


Western Blot: B Raf Antibody (1D2) [NBP2-02260] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B Raf (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: B Raf Antibody (1D2) [NBP2-02260] Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.
Flow Cytometry: B Raf Antibody (1D2) [NBP2-02260] - Analysis of Jurkat cells, using anti-B Raf antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: B Raf Antibody (1D2) [NBP2-02260] - Analysis of Hela cells, using anti-B Raf antibody, (Red) compared to a nonspecific negative control antibody (Blue).

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B-Raf Antibody (OTI1D2) Summary

Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
Immunogen affinity purified
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  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
Positive Control
B-Raf Lysate (NBP2-66112)

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for B-Raf Antibody (OTI1D2)

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109


This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects,mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for B-Raf Antibody (NBP2-02260)

Discover related pathways, diseases and genes to B-Raf Antibody (NBP2-02260). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for B-Raf Antibody (NBP2-02260)

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Blogs on B-Raf.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.

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Gene Symbol BRAF