B-Raf Antibody (4G6)

Images

 
Western Blot: B Raf Antibody (4G6) [NBP1-47670] - Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-B Raf monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-B Raf mouse monoclonal antibody.
Western Blot: B Raf Antibody (4G6) [NBP1-47670] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B Raf (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded breast tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded colon tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded endometrium tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded lung tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded lymphoma tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded Ovary tissue using anti-B Raf mouse monoclonal antibody.
Immunohistochemistry-Paraffin: B Raf Antibody (4G6) [NBP1-47670] - Staining of paraffin-embedded thyroid tissue using anti-B Raf mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47670
    • Availability
      Product Discontinued

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B-Raf Antibody (4G6) Summary

Immunogen
Protein expressed in 293T cell transfected with human BRAF expression vector
Specificity
This antibody is specific for Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRAF
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Primate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for B-Raf Antibody (4G6)

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this ge

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics Tool for B-Raf Antibody (NBP1-47670)

Discover related pathways, diseases and genes to B-Raf Antibody (NBP1-47670). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B-Raf Antibody (NBP1-47670)

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Pathways for B-Raf Antibody (NBP1-47670)

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PTMs for B-Raf Antibody (NBP1-47670)

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Research Areas for B-Raf Antibody (NBP1-47670)

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Blogs on B-Raf.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.

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Bioinformatics

Gene Symbol BRAF
Entrez