AZ1 Antibody

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Independent Antibodies: Western Blot: AZ1 Antibody [NB100-68225] - Detection of Human CEP131/AZ1 on HeLa whole cell lysate using NB100-68225. CEP131/AZ1 was also immunoprecipitated by rabbit anti-CEP131/AZ1 ...read more
Immunocytochemistry/ Immunofluorescence: AZ1 Antibody [NB100-68225] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:200.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

AZ1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEP131
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunofluorescence (ICC)- Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AZ1 Antibody

  • 5-azacytidine induced 1
  • 5-azacytidine-induced protein 1
  • AZ1
  • centrosomal protein 131 kDa
  • centrosomal protein of 131 kDa
  • CEP131
  • KIAA1118
  • pre-acrosome localization protein 1
  • ZA1

Background

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Additional AZ1 Products

Array NB100-68225

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Bioinformatics

Gene Symbol CEP131
Entrez
Uniprot