Axin-1 Antibody (1A4)

Images

 
Western Blot: Axin-1 Antibody (1A4) [NBP1-74031] - Expressed in AGS and SW480 cell lines. Immunoprecipitation (IP) and western blot experiments were performed to evaluate the Axin1 antibodys specificity. IP sample and ...read more
Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody (1A4) [NBP1-74031] - staining of human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Axin-1 Antibody (1A4) Summary

Immunogen
Recombinant human Axin1 (546-752aa) purified from E. coli
Specificity
Anti-human Axin1 mAb, clone 1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Axin1 protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AXIN1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The antibody has been tested by ELISA, Western blot, Immunofluorescence, and Immunoprecipitation analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Axin-1 Antibody (1A4)

  • axin 1
  • Axin1
  • Axin-1
  • AXINaxis inhibitor 1
  • Axis inhibition protein 1
  • fused, mouse, homolog of
  • hAxin
  • MGC52315

Background

Axin was originally identified as the product of the mouse gene called "fused" or fu (renamed Axin), and has since been shown to play a crucial role in controlling axis formation during embryonic development. Axin1 is characterized to be a tumor suppressor and numerous gene mutations have been identified throughout Axin1 from a number of different cancers. Axin can identify in a mouse mutant strain, created by random integration of a transgene. Also, Axin1 plays an important role in the formation of the embryonic neural axis. Recently, it was reported that Axin is found in mitotic spindles and centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-74031AF405
Alexa Fluor 488 Labeled NBP1-74031AF488
Alexa Fluor 647 Labeled NBP1-74031AF647
Alexa Fluor 700 Labeled NBP1-74031AF700
DyLight 350 Labeled NBP1-74031UV
DyLight 405 Labeled NBP1-74031V
DyLight 488 Labeled NBP1-74031G
DyLight 680 Labeled NBP1-74031FR
DyLight 755 Labeled NBP1-74031IR

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Bioinformatics

Gene Symbol AXIN1
Entrez
Uniprot