Avidin Antibody [Biotin]

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Western Blot: Avidin Antibody [Biotin] [NB120-7235] - Lane 1: Avidin. Lane 2: None. Load: 100 ng per lane. Used at a dilution of 1:1000 for 1H at RT.

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

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Avidin Antibody [Biotin] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Avidin [Hen Egg White] (Native Protein)
Specificity
Purity/Specifity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Readout System

Reactivity Notes

No cross reactivity occurs against Streptavidin.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 100 ul deionized water

Alternate Names for Avidin Antibody [Biotin]

  • AVD
  • Avidin
  • LOC396260

Background

The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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