ATR Antibody

Product Discontinued

ATR Antibody Summary

• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
• Specificity: This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:15000-1:70000
  • Western Blot 1:1000-1:5000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 301 kDa in size corresponding - ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, u2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Reactivity Notes

Reactive in human, mouse, rat, monkey, dog, fish, Xenopus

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.28 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATR Antibody

• ataxia telangiectasia and Rad3 related
• Ataxia telangiectasia and Rad3-related protein
• ATR
• EC 2.7.11.1
• FRAP-related protein 1
• FRP1
• FRP1FRAP-related protein-1
• MEC1
• protein kinase ATR
• Rad3 related protein
• SCKL1
• SCKLMEC1, mitosis entry checkpoint 1, homolog
• serine/threonine-protein kinase ATR

Background

Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATR