ATR Antibody

Product Discontinued

ATR Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1675 and 1725 of human Ataxia Telangiectasia and Rad3-related using the numbering given in entry NP_001175.1 (GeneID 545).
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:15000-1:30000
• Theoretical MW: 301 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as secondary antibodies: NB100-305, NB100-55324, NB100-1803, NB100-224, NB100-41429, NB100-381, NB100-464, NB100-2241, NB500-158, NB100-2350, NB100-204, NB100-206, NB110-40543

Alternate Names for ATR Antibody

• ataxia telangiectasia and Rad3 related
• Ataxia telangiectasia and Rad3-related protein
• ATR
• EC 2.7.11.1
• FRAP-related protein 1
• FRP1
• FRP1FRAP-related protein-1
• MEC1
• protein kinase ATR
• Rad3 related protein
• SCKL1
• SCKLMEC1, mitosis entry checkpoint 1, homolog
• serine/threonine-protein kinase ATR

Background

Ataxia and Rad3-related protein kinase (ATR) is a direct upstream activator of Chk1. This suggests that ATR plays a role in sensing DNA damage and initiating a subsequent protein kinase cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATR Antibody (NB100-322)(6)

Publications using NB100-322

• Li R, Yang Y-G, Gao Y et al. A distinct response to endogenous DNA damage in the development of Nbs1-deficient cortical neurons. Cell Research. 2012-01-03 [PMID: 22212482]
• Yang YN, Chou KM, Pan W et al. Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036 Cancer Lett 2011-10-01 [PMID: 21684680] (WB, Human)
• Mohni KN, Livingston CM, Cortez D et al. ATR and ATRIP are recruited to herpes simplex virus type 1 replication compartments even though ATR signaling is disabled. J Virol 2010-12-01 [PMID: 20861269]
• Singh, R et al. Silibinin suppresses in vivo growth of human prostate carcinoma PC-3 tumor xenograft. Carcinogenesis;28(12):2567-74. 2007-12-01 [PMID: 17916909]
• Alberstein M, Amit M, Vaknin K et al. Regulation of transcription of the RNA splicing factor hSlu7 by Elk-1 and Sp1 affects alternative splicing. RNA 2007-11-01 [PMID: 17804646]
• Tyagi, A et al. Silibinin activates p53-caspase 2 pathway and causes caspase-mediated cleavage of Cip1/p21 in apoptosis induction in bladder transitional-cell papilloma RT4 cells: evidence for a regulatory loop between p53 and caspase 2. Carcinogenesis;27(11):2269-80. 2006-11-01 [PMID: 16777994]

Bioinformatics

• Gene Symbol: ATR
• Entrez:
  • 545(Human)
  • 245000(Mouse)
• Uniprot:
  • Q13535()