ATP6V1B1 Antibody (2D2)

Images

 
Western Blot: ATP6V1B1 Antibody (2D2) [NBP2-00572] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: ATP6V1B1 Antibody (2D2) [NBP2-00572] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1.
Flow Cytometry: ATP6V1B1 Antibody (2D2) [NBP2-00572] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody, and then ...read more
Flow Cytometry: ATP6V1B1 Antibody (2D2) [NBP2-00572] - Analysis of Hela cells, using anti-ATP6V1B1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ATP6V1B1 Antibody (2D2) [NBP2-00572] - Analysis of Jurkat cells, using anti-ATP6V1B1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00572
    • Availability
      Product Discontinued

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ATP6V1B1 Antibody (2D2) Summary

Immunogen
Full length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ATP6V1B1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP6V1B1 Antibody (2D2)

  • ATP6B158kD subunit
  • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
  • Endomembrane proton pump 58 kDa subunit
  • H+-ATPase beta 1 subunit
  • vacuolar proton pump 3
  • Vacuolar proton pump subunit B 1
  • vacuolar proton pump, subunit 3
  • VATBMGC32642
  • V-ATPase B1 subunit
  • V-ATPase subunit B 1
  • Vma2
  • V-type proton ATPase subunit B, kidney isoform

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP6V1B1
Entrez