ATP Citrate Lyase Antibody (3G8)

Images

 
Western Blot: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP Citrate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP Citrate Lyase.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded bladder tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded colon tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded endometrium tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Kidney tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded liver tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded lung tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded prostate tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (3G8) [NBP2-02410] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ATP Citrate Lyase Antibody (3G8) Summary

Immunogen
Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACLY
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP Citrate Lyase Antibody (3G8)

  • ACLEC 2.3.3.8
  • ATP citrate lyase
  • ATP citrate synthase
  • ATP-citrate (pro-S-)-lyase
  • ATP-citrate synthase
  • ATPCL
  • Citrate cleavage enzyme
  • CLATP

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACLY
Entrez