ATM [p Ser1981] Antibody (10H11.E12)

Product Discontinued

ATM [p Ser1981] Antibody (10H11.E12) Summary

• Immunogen: Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]
• Modification: p Ser1981
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATM
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:1000
• Application Notes: This ATM [p Ser1981] (10H11.E12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a single band is seen at ~370 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 370 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for ATM [p Ser1981] Antibody (10H11.E12)

• AT mutated
• A-T mutated
• AT1
• ATA
• ataxia telangiectasia mutated (includes complementation groups A, C and D)
• ataxia telangiectasia mutated
• ATC
• ATD
• ATDC
• ATE
• ATM serine/threonine kinase
• ATM
• DKFZp781A0353
• EC 2.7.11.1
• MGC74674
• serine-protein kinase ATM
• TEL1
• TEL1, telomere maintenance 1, homolog
• TELO1
• TPLL

Background

ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.

References

1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATM Antibody (NB100-307)(5)

Publications using NB100-307

• Cao K, Riley JS, Heilig R et al. Mitochondrial dynamics regulate genome stability via control of caspase-dependent DNA damage Developmental cell 2022-04-14 [PMID: 35447090] (ICC/IF, Human)
• Tesson M, Anselmi G, Bell C, Mairs R. Cell cycle specific radiosensitisation by the disulfiram and copper complex Oncotarget 2017-09-12 [PMID: 29029481] (WB, Human)
• Carruthers R, Ahmed SU, Strathdee K et al. Abrogation of radioresistance in glioblastoma stem-like cells by inhibition of ATM kinase. Molecular Oncology. 2014-08-24 [PMID: 25205037]
• Chu, PC et al. Silencing of p29 Affects DNA Damage Responses with UV Irradiation. Cancer Res. 66: 8484-8491. 2006-01-01 [PMID: 16951160]
• Bartkova J, Bakkenist CJ, Rajpert-De Meyts E et al. ATM activation in normal human tissues and testicular cancer. Cell Cycle 2005-06-01 [PMID: 15846060]

Review for ATM Antibody (NB100-307) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/14/2014

Summary

• Application: Western Blot
• Sample Tested: UVW glioma cell line whole cell lysate
• Species: Human
• Lot: A3

FAQs for ATM Antibody (NB100-307). (Showing 1 - 2 of 2 FAQs).

1. What is the theoretical molecular weight for your ATM antibodies?
   • The theoretical molecular weight for our ATM antibodies is 351 kDa.
2. Is it possible to let me know what is the difference between NB100-307 and NB100-306 which are the same clone?
   • NB100-307 is unpurified mouse ascites, while NB100-306 is the purified version of the same antibody. They should detect similarly, with the purified one providing less background, and is tested in more applications.

Bioinformatics

• Gene Symbol: ATM
• Entrez:
  • 472(Human)
• Uniprot:
  • Q13315()