ATG7 Antibody

Images

 
Western Blot: ATG7 Antibody [NB110-74811] - WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight.

Product Details

Summary
Product Discontinued
View other related ATG7 Primary Antibodies

Order Details


    • Catalog Number
      NB110-74811
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

ATG7 Antibody Summary

Immunogen
A synthetic peptide as a part of human, rat, mouse autophagy-related protein 7 (ATG7, APG7-like, APG7L, hAGP7) conjugated to immunogenic carrier protein has been used as the immunogen.
Specificity
ATG7.
Clonality
Polyclonal
Host
Rabbit
Gene
ATG7
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:2000
Application Notes
Although not tested this antibody may be useful in IHC-Paraffin/frozen. Western Blot (PMID: 22395361)The optimal dilution should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27231155).
Positive Control
ATG7 Lysate (NBL1-07800)
Publications
Read Publications using
NB110-74811 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.15 ml sterilized water.

Alternate Names for ATG7 Antibody

  • APG7 autophagy 7-like (S. cerevisiae)
  • APG7
  • APG7L
  • APG7-LIKE
  • ATG12-activating enzyme E1 ATG7
  • ATG7 autophagy related 7 homolog (S. cerevisiae)
  • ATG7
  • Autophagy-related protein 7
  • DKFZp434N0735
  • GSA7
  • hAGP7
  • ubiquitin activating enzyme E1-like protein
  • Ubiquitin-activating enzyme E1-like protein
  • ubiquitin-like modifier-activating enzyme ATG7

Background

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.; FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.; SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.; SUBCELLULAR LOCATION: Cytoplasm.; TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.; DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-53818
Species: Hu, Mu, Rt, Po, Bv, Pm, Xp, Ze
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, PLA
NB500-249
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB100-2331
Species: Hu, Mu, Rt, Ca, Fi, Pl, Ze
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB
NBP1-88883
Species: Hu
Applications: WB, IHC, IHC-P
H00008878-M01
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
NBP2-15501
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
NBP1-55202
Species: Hu
Applications: WB, IHC, IHC-P
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
NBP2-01083
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85091
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37399
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
NBP2-02627
Species: Hu, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-19151
Species: Hu, Ma
Applications: WB, IHC-P, IP
NBP2-24738
Species: Hu, Mu, Pm
Applications: WB, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NB110-74811
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for ATG7 Antibody (NB110-74811)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species

Reviews for ATG7 Antibody (NB110-74811) (0)

There are no reviews for ATG7 Antibody (NB110-74811). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG7 Antibody (NB110-74811) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ATG7 Products

Bioinformatics Tool for ATG7 Antibody (NB110-74811)

Discover related pathways, diseases and genes to ATG7 Antibody (NB110-74811). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATG7 Antibody (NB110-74811)

Discover more about diseases related to ATG7 Antibody (NB110-74811).
 

Pathways for ATG7 Antibody (NB110-74811)

View related products by pathway.

PTMs for ATG7 Antibody (NB110-74811)

Learn more about PTMs related to ATG7 Antibody (NB110-74811).
 

Research Areas for ATG7 Antibody (NB110-74811)

Find related products by research area.

Blogs on ATG7.

ATG4B - a cysteine protease involved in autophagosome elongation
Autophagy can be broken down into 4 main stages: phagophore nucleation, autophagosome elongation, autophagosome docking and fusion with a lysosome, and vesicle breakdown and degradation. ATG4B is one of four ATG4 homologs (ATG4A, ATG4B, ATG4C, and...  Read full blog post.

ATG7 - an E1 enzyme for the ubiquitin-like autophagy proteins
Autophagy is an essential cellular process that maintains homeostasis through the degradation and recycling of cytoplasmic organelles and macromolecules. Substrates targeted for autophagy are engulfed in a double-membrane structure called the auto...  Read full blog post.

ATG12 - a ubiquitin-like protein essential for autophagosome assembly
Atg12 is a ubiquitin-like protein that plays an essential role in cellular homeostasis by regulating the degradation and recycling of cytoplasmic organelles and macromolecules. Atg12 is one of two ubiquitin-like protein systems that is required du...  Read full blog post.

Essential to Death: ATG5 (autophagy protein 5, apoptosis-specific protein ASP)
The ATG5 protein belongs to the ATG autophagy regulator family. This family controls the highly conserved cell's homeostatic response to a wide variety of both self- and foreign-originating cellular stimuli. ATG5 itself is ubiquitously expressed in mo...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ATG7 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ATG7
Entrez
Uniprot