ATG4B Antibody

Product Discontinued

ATG4B Antibody Summary

• Immunogen: A synthetic peptide (CLERFFDSEDED) corresponding to the C-terminus of human ATG4B protein conjugated to Blue Carrier Protein has been used as the immunogen.
• Specificity: IHC, WB and ELISA confirmed the specificity for ATG4B.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2000
  • Immunohistochemistry 1:100-1:2000
  • Western Blot 1:100-1:2000
• Control:

  ATG4B Overexpression Lysate

Reactivity Notes

Human, rat. The peptide is homologous with the corresponding sequence derived from of ATG4B protein in mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: IgG purified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water.

Alternate Names for ATG4B Antibody

• APG4 autophagy 4 homolog B (S. cerevisiae)
• APG4 autophagy 4 homolog B
• Apg4B
• ATG4 autophagy related 4 homolog B (S. cerevisiae)
• ATG4B
• AUTL1
• AUTL1MGC1353
• AUT-like 1 cysteine endopeptidase
• Autophagin-1
• Autophagy-related cysteine endopeptidase 1
• Autophagy-related protein 4 homolog B
• cysteine protease ATG4B
• DKFZp586D1822
• EC 3.4.22
• EC 3.4.22.-
• hAPG4B
• KIAA0943

Background

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATG4B Antibody (R-157-100)(6)

Publications using R-157-100

• Kumanomidou T et al. J. Mol. Biol. 355:612-618. 2006-01-01 [PMID: 16325851]
• Sugawara K et al. J. Biol. Chem. 280:40058-40065. 2005-01-01 [PMID: 16183633]
• Kabeya Y et al. J. Cell Sci. 117:2805-2812. 2004-01-01 [PMID: 15169837]
• Ota,T. Nat. Genet. 36 (1), 40-45. 2004-01-01 [PMID: 14702039]
• Marino G, Uria JA, Puente XS et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. J Biol Chem 2003-02-01 [PMID: 12446702]
• Nagase T, Ishikawa K, Suyama M et al. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res 1999-02-26 [PMID: 10231032]

Bioinformatics

• Gene Symbol: ATG4B
• Entrez:
  • 23192 (Human)
  • 316640 (Rat)
• Uniprot:
  • Q9Y4P1(Human)