ATG3/APG3 Antibody

Images

 
Western Blot: ATG3/APG3 Antibody [NBP1-77168] - Mouse kidney tissue Lysate with ATG3 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: ATG3/APG3 Antibody [NBP1-77168] - Immunofluorescence of ATG3 in human kidney with ATG3 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP1-77168] - Human kidney tissue with ATG3 antibody at 5 ug/mL.

Product Details

Summary
Product Discontinued
View other related ATG3/APG3 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-77168
    • Availability
      Product Discontinued

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ATG3/APG3 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3. The immunogen is located within amino acids 180 - 230 of ATG3.
Clonality
Polyclonal
Host
Rabbit
Gene
ATG3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG3/APG3 Antibody

  • APG3 autophagy 3-like (S. cerevisiae)
  • APG3
  • APG3L
  • APG3-LIKE
  • Apg3p
  • ATG3 autophagy related 3 homolog (S. cerevisiae)
  • ATG3
  • Autophagy-related protein 3
  • DKFZp564M1178
  • EC 6.3.2.-
  • FLJ22125,2610016C12Rik
  • hApg3
  • MGC15201
  • PC3-96
  • Protein PC3-96
  • ubiquitin-like-conjugating enzyme ATG3

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Diseases for ATG3/APG3 Antibody (NBP1-77168)

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Bioinformatics

Gene Symbol ATG3
Entrez
Uniprot