ATG3/APG3 Antibody (3H2)

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Western Blot: Apg3 Antibody (3H2) [NBP2-01083] Analysis of extracts (35ug) from 9 different cell lines by using anti-Apg3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: Apg3 Antibody (3H2) [NBP2-01083] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Apg3.
Immunohistochemistry-Paraffin: Apg3 Antibody (3H2) [NBP2-01083] - Staining of paraffin-embedded Human tonsil using anti-Apg3 mouse monoclonal antibody.
Flow Cytometry: Apg3 Antibody (3H2) [NBP2-01083] - Analysis of Hela cells, using anti-Apg3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: Apg3 Antibody (3H2) [NBP2-01083] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apg3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: Apg3 Antibody (3H2) [NBP2-01083] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Apg3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Apg3 Antibody (3H2) [NBP2-01083] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Apg3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Apg3 Antibody (3H2) [NBP2-01083] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Apg3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Apg3 Antibody (3H2) [NBP2-01083] - Staining of paraffin-embedded Human Kidney tissue using anti-Apg3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ATG3/APG3 Antibody (3H2) Summary

Immunogen
Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATG3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATG3/APG3 Antibody (3H2)

  • APG3 autophagy 3-like (S. cerevisiae)
  • APG3
  • APG3L
  • APG3-LIKE
  • Apg3p
  • ATG3 autophagy related 3 homolog (S. cerevisiae)
  • ATG3
  • Autophagy-related protein 3
  • DKFZp564M1178
  • EC 6.3.2.-
  • FLJ22125,2610016C12Rik
  • hApg3
  • MGC15201
  • PC3-96
  • Protein PC3-96
  • ubiquitin-like-conjugating enzyme ATG3

Background

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATG3
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