ATF2 [p Thr71, p Thr53] Antibody

Images

 
Western Blot: ATF2 [phospho Thr71/Thr53] Antibody [NB100-92477] - (WB) analysis of p-ATF2 (T69/51) antibody in extracts from LOVO cells
Immunohistochemistry-Paraffin: ATF2 [phospho Thr71/Thr53] Antibody [NB100-92477] - Paraffin-embedded human breast carcinoma tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92477
    • Availability
      Product Discontinued

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ATF2 [p Thr71, p Thr53] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 71.
Modification
p Thr71, p Thr53
Clonality
Polyclonal
Host
Rabbit
Gene
ATF2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:500-1:200
Application Notes
Western blot (WB) analysis of p-ATF2 (T71/53) pAb in extracts from HeLa cells treated with UV..
Immunohistochemistry (IHC) analyzes of p-ATF2 (T71/53) pAb in paraffin-embedded human breast carcinoma tissue
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF2 [p Thr71, p Thr53] Antibody

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular CIS acting promotor and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATF2
Entrez