ATF2 Antibody

Images

 
Western Blot: ATF2 Antibody [NB100-91668] - Extracts from LOVO cells.
Immunohistochemistry-Paraffin: ATF2 Antibody [NB100-91668] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related ATF2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91668
    • Availability
      Product Discontinued

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ATF2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 30-80 of Human ATF2.
Specificity
This antibody detects endogenous levels of ATF2 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
ATF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:50-1:200
Application Notes
Western blot (WB) analysis of ATF2 (V63/45) pAb in extracts from LOVO cells.
Immunohistochemistry (IHC) analyzes of ATF2 (V63/45) pAb in paraffin-embedded human breast carcinoma tissue..

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF2 Antibody

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATF2
Entrez
Uniprot