ATF1 Antibody

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Western Blot: ATF1 Antibody [NBP1-52645] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-ATF1 antibody used for WB at ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-52645
    • Availability
      Product Discontinued

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ATF1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human Activating Transcription Factor 1 using the numbering given in entry NP_005162.1 (GeneID 466).
Clonality
Polyclonal
Host
Rabbit
Gene
ATF1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 mcg/mg

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF1 Antibody

  • activating transcription factor 1FUS/ATF-1
  • ATF1
  • cAMP-dependent transcription factor ATF-1
  • EWS-ATF1
  • Protein TREB36
  • TREB36
  • TREB36cyclic AMP-dependent transcription factor ATF-1

Background

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATF1
Entrez