ATF1 Antibody

Images

 
Western Blot: ATF1 Antibody [NBP1-51289] - Analysis of ATF1 (I57) pAb in extracts from COLO cells.
Immunohistochemistry-Paraffin: ATF1 Antibody [NBP1-51289] - Analyzes of ATF1 (I57) pAb in paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51289
    • Availability
      Product Discontinued

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ATF1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 20-70 of Human ATF1.
Specificity
ATF-1 (I57) pAb detects endogenous levels of ATF-1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
ATF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of ATF-1 (I57) pAb in extracts from COLO cells.
Immunohistochemistry (IHC) analyzes of ATF-1 (I57) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF1 Antibody

  • activating transcription factor 1FUS/ATF-1
  • ATF1
  • cAMP-dependent transcription factor ATF-1
  • EWS-ATF1
  • Protein TREB36
  • TREB36
  • TREB36cyclic AMP-dependent transcription factor ATF-1

Background

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATF1
Entrez