Aspartate Aminotransferase Antibody

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Western Blot: Aspartate Aminotransferase Antibody [NB100-77335] - Analysis of Aspartate Aminotransferase (aa 22-35) using NB100-77335 at 0.5ug/ml in Human Liver lysate (35ug protein in RIPA buffer). Primary incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Aspartate Aminotransferase Antibody Summary

Immunogen
Peptide with sequence C-TADFREDPDPRKVN corresponding to internal region according to NP_002070.1.
Localization
Cytoplasmic and Mitochondrial
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GOT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 40 kDa band observed in human liver lysates is reported in scientific literature (PMID: 2185471).
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Aspartate Aminotransferase Lysate (NBL1-11207)
Publications
Read Publication using NB100-77335.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aspartate Aminotransferase Antibody

  • aspartate aminotransferase, cytoplasmic
  • EC 2.6.1.1
  • GIG18
  • Glutamate oxaloacetate transaminase 1
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • growth-inhibiting protein 18
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aspartate Aminotransferase Antibody (NB100-77335)(1)


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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GOT1
Entrez
Uniprot