ASK1 Antibody

Images

 
Western Blot: ASK1 Antibody [NBP1-77032] - SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: ASK1 Antibody [NBP1-77032] - Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: ASK1 Antibody [NBP1-77032] - A431 cells with ASK1 antibody at 10 ug/ml.

Product Details

Summary
Product Discontinued
View other related ASK1 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-77032
    • Availability
      Product Discontinued

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ASK1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. The sequence is different from that of mouse by last two amino acids. The immunogen is located within the last 50 amino acids of ASK1.
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
ASK1 antibody can be used for detection of ASK1 by Western blot at 1:500 to 1:1000 dilution. A 155 kDa band can be detected.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASK1 Antibody

  • Apoptosis signal-regulating kinase 1
  • ASK1
  • ASK-1
  • ASK1MEKK 5
  • EC 2.7.11
  • MAP/ERK kinase kinase 5
  • MAP3K5
  • MAPK/ERK kinase kinase 5
  • MAPKKK5EC 2.7.11.25
  • MEK kinase 5
  • MEKK5
  • MEKK5apoptosis signal regulating kinase 1
  • mitogen-activated protein kinase kinase kinase 5

Background

Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional ASK1 Products

Bioinformatics Tool for ASK1 Antibody (NBP1-77032)

Discover related pathways, diseases and genes to ASK1 Antibody (NBP1-77032). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ASK1 Antibody (NBP1-77032)

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Pathways for ASK1 Antibody (NBP1-77032)

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Research Areas for ASK1 Antibody (NBP1-77032)

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Blogs on ASK1.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

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Bioinformatics

Gene Symbol MAP3K5
Entrez
Uniprot