ASK1 Antibody

Images

 
Western Blot: ASK1 Antibody [NB100-91664] - Extracts from MDA-MB-435 cells.
Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-91664] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related ASK1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91664
    • Availability
      Product Discontinued

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ASK1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 43-90 of Human ASK1.
Specificity
This antibody detects endogenous levels of ASK1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of ASK1 (T77) pAb in extracts from MDA-MB-435 cells.
Immunohistochemistry (IHC) analyzes of ASK1 (T77) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ASK1 Lysate (NBL1-12856)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASK1 Antibody

  • Apoptosis signal-regulating kinase 1
  • ASK1
  • ASK-1
  • ASK1MEKK 5
  • EC 2.7.11
  • MAP/ERK kinase kinase 5
  • MAP3K5
  • MAPK/ERK kinase kinase 5
  • MAPKKK5EC 2.7.11.25
  • MEK kinase 5
  • MEKK5
  • MEKK5apoptosis signal regulating kinase 1
  • mitogen-activated protein kinase kinase kinase 5

Background

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ASK1 Antibody (NB100-91664)

Discover related pathways, diseases and genes to ASK1 Antibody (NB100-91664). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for ASK1 Antibody (NB100-91664)

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Blogs on ASK1.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

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Bioinformatics

Gene Symbol MAP3K5
Entrez