ASCL2/Mash2 Antibody [Alexa Fluor® 350]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

ASCL2/Mash2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human ASCL2/Mash2
  Met1-Ala49
  Accession # Q99929
• Specificity: Detects human ASCL2/Mash2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ASCL2 (Achaete-scute like 2; also HASH2, bHLHa45 and Mash2) is a class II member of the HLH family of transcription factors. Its predicted MW is 20 kDa. ASCL2 shows restricted expression, being limited to intestinal Lgr5⁺ stem cells and first trimester placental cytotrophoblasts. In the intestine, ASCL2 is under the control of Wnts and serves to maintain an epithelium stem cell pool. In the placenta, ASCL2 acts to maintain the pool of cytotrophoblasts at the expense of synctiotrophoblasts, thus promoting placental growth. Human ASCL2 is 193 amino acids (aa) in length. It contains one poly-Arg motif (aa 36-39), a DNA binding sequence (aa 53-63) and an HLH domain (aa 64-103). ASCL2 forms heterodimers with daughterless homologs (E22, E2A and HEB). Over aa 1-49, human ASCL2 shares 59% aa identity with mouse Mash2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.