ASC/TMS1 Antibody

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Western Blot: ASC/TMS1 Antibody [NBP2-15485] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody [NBP2-15485] - HCT 116 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: TMS1 protein stained by TMS1 antibody [N1C3] diluted at ...read more
Western Blot: ASC/TMS1 Antibody [NBP2-15485] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of 3xFlag-human TMS1-transfected 293T cells 12 % SDS-PAGE TMS1 antibody dilution: 1:20000.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ASC/TMS1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TMS1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Monkey (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYCARD
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

  • ASC
  • ASCMGC10332
  • CARD5
  • CARD5TMS
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain containing
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS1
  • TMS-1
  • TMS1apoptosis-associated speck-like protein containing a CARD

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Research Areas for ASC/TMS1 Antibody (NBP2-15485)

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Blogs on ASC/TMS1.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Bioinformatics

Gene Symbol PYCARD
Entrez
Uniprot

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