ASC/TMS1 Antibody (3E9)

Images

 
Western Blot: ASC/TMS1 Antibody (3E9) [NBP2-03818] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ASC/TMS1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (3E9) [NBP2-03818] - Staining of paraffin-embedded Human liver tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Flow Cytometry: ASC/TMS1 Antibody (3E9) [NBP2-03818] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ASC/TMS1 antibody, and then ...read more
Western Blot: ASC/TMS1 Antibody (3E9) [NBP2-03818] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASC/TMS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (3E9) [NBP2-03818] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (3E9) [NBP2-03818] - Staining of paraffin-embedded Human colon tissue using anti-ASC/TMS1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
3E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ASC/TMS1 Antibody (3E9) Summary

Immunogen
Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Application Notes
For IHC-P, antigen retrieval is recommended with 10mM citric buffer, pH6.0, 100C for 10min.
Publications
Read Publication using NBP2-03818.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody (3E9)

  • ASC
  • ASCMGC10332
  • CARD5
  • CARD5TMS
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain containing
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS1
  • TMS-1
  • TMS1apoptosis-associated speck-like protein containing a CARD

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASC/TMS1 Antibody (NBP2-03818)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ASC/TMS1 Antibody (NBP2-03818). (Showing 1 - 1 of 1 FAQ).

  1. Please advise the antigen retrieval conditions/procedure that you recommend.
    • For this item our lab recommends performing heat induced antigen retrieval with 10mM citric buffer, pH6.0, 100C for 10min.

Secondary Antibodies

 

Isotype Controls

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Research Areas for ASC/TMS1 Antibody (NBP2-03818)

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Blogs on ASC/TMS1.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Bioinformatics

Gene Symbol PYCARD
Entrez