Armenian Hamster IgG Native Protein

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Product Details

Summary
Reactivity ArHaSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IP
Concentration
LYOPH

Order Details

Armenian Hamster IgG Native Protein Summary

Description
Native Armenian Hamster IgG protein.

Source: Armenian Hamster Serum

Preparation
Method
ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG. Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunoelectrophoresis (IEP)
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

For extended storage, aliquot reconstituted liquid and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use.

Background

Armenian Hamster isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In hamster there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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