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ARMC8 RNAi

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Summary
Reactivity HuSpecies Glossary
Applications RNAi, RNAi SP

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ARMC8 RNAi Summary

Specificity
Homo sapiens armadillo repeat containing 8 (ARMC8), transcript variant 2, mRNA
Gene
ARMC8

Applications/Dilutions

Application Notes
This RNAi causes protein knockdown.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
DEPC-treated Water

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARMC8 RNAi

  • armadillo repeat containing 8
  • armadillo repeat-containing protein 8
  • DKFZP434A043
  • HSPC056
  • MGC10058
  • MGC4880

Background

Position of the Chimera RNAi. The related RNAi products listed were designed from different accesion number but sharing the same RNAi sequence. Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene.  The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.  Chimera RNAi has many advantages over the conventional siRNAs.  First, it has been demonstrated to have reliable knock-down for over 10,000 human genes.  Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence.  Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected.  Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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Western Blot: RMND5A Antibody [NBP1-92337] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: RMND5A Antibody [NBP1-92337] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Western Blot: RMND5A Antibody [NBP1-92337] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
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HGS Antibody
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Western Blot: HGS Antibody [NBP1-83202] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: HGS Antibody [NBP1-83202] - Staining of human cell line U-2 OS shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
C17orf39 Antibody
NBP1-91717
Immunocytochemistry/Immunofluorescence: C17orf39 Antibody [NBP1-91717] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: C17orf39 Antibody [NBP1-91717] - Staining of human testis shows cytoplasmic positivity in cells of seminiferous ducts.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
RMND5B Antibody
NBP2-13234
Western Blot: RMND5B Antibody [NBP2-13234] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: RMND5B Antibody [NBP2-13234] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RMND5B Antibody [NBP2-13234] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
ROR
gamma/RORC/NR1F3 Anti
NBP2-24503
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ROR gamma at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - IHC analysis of a formalin fixed paraffin embedded tissue section of human lymph node using 5ug/ml concentration of anti- ROR-gamma/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated mainly a nuclear staining in a subset of cells and a weak to cytoplasmic staining was also observed in some cells.
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(1 Publication)
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
WDR26 Antibody
NBP1-83628
Western Blot: WDR26 Antibody [NBP1-83628] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/Immunofluorescence: WDR26 Antibody [NBP1-83628] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: WDR26 Antibody [NBP1-83628] - Staining of human testis shows high expression.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
TWA1 Antibody
NBP1-32596
Western Blot: TWA1 Antibody [NBP1-32596] - (30 ug whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000
Immunocytochemistry/Immunofluorescence: TWA1 Antibody [NBP1-32596] - Paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
ATN1 Antibody
NBP1-90044
Immunocytochemistry/Immunofluorescence: ATN1 Antibody [NBP1-90044] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Staining in human cerebral cortex and liver tissues using anti-ATN1 antibody. Corresponding ATN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
(1 Publication)
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EMP/MAEA Antibody
(730340)
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NR8383 rat alveolar macrophage cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat EMP/MAEA Monoclonal Antibody (Catalog # MAB7288) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for EMP/MAEA at approximately 36-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Species: Hu, Mu, Rt
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ZNF645 Antibody
NBP2-21037
Western Blot: ZNF645 Antibody [NBP2-21037] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: ZNF645 Antibody [NBP2-21037] - Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, IHC, IHC-P
Mkln1 Antibody
NBP1-80655
Immunohistochemistry-Paraffin: Mkln1 Antibody [NBP1-80655] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
HARS Antibody
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Western Blot: HARS Antibody [NBP1-89490] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: HARS Antibody [NBP1-89490] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: HARS Antibody [NBP1-89490] - Staining of human kidney shows positivity in cells in tubules.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
RANBP9 Antibody
NBP2-15087
Western Blot: RANBP9 Antibody [NBP2-15087] - Sample (30 ug of whole cell lysate) A: Hela B: NT2D1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: RANBP9 Antibody [NBP2-15087] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Species: Hu, Mu
Applications: WB
MUL1 Antibody
NBP2-31363
Western Blot: MUL1 Antibody [NBP2-31363] - Western blot analysis of K562 (A), Rt4 (B), and SH-SY5Y (C) cell lysate using MUL1 antibody at a concentration of 2 ug/ml.
Immunocytochemistry/Immunofluorescence: MUL1 Antibody [NBP2-31363] - MUL1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used.
Western Blot: MUL1 Antibody [NBP2-31363] - WB analysis of MUL1 protein in lysate of human liver and partial recombinant MUL1 protein by using the primary antibody concentration of 2ug/ml and 0.5 ug/ml respectively.
Species: Hu
Applications: WB, ICC/IF
SFRS5 Antibody
NBP1-92381
Western Blot: SFRS5 Antibody [NBP1-92381] - Analysis in human cell line HeLa.
Immunocytochemistry/Immunofluorescence: SFRS5 Antibody [NBP1-92381] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.
Immunohistochemistry-Paraffin: SFRS5 Antibody [NBP1-92381] - Staining in human fallopian tube and pancreas tissues. Corresponding SRSF5 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
TRIM50 Antibody
NBP1-81988
Immunohistochemistry-Paraffin: TRIM50 Antibody [NBP1-81988] - Staining of human bone marrow shows strong cytoplasmic positivity in a subset of bone marrow poietic cells.
Species: Hu
Applications: IHC, IHC-P
Catenin alpha 1
Antibody
NBP2-15723
Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Catenin alpha 1 Antibody [NBP2-15723] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. Green: alpha Catenin antibody [N1N3] diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Ran Antibody
(8D1A6)
NBP2-61832
Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC
beta-Catenin
Antibody (12F7)
NBP1-54467
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - IHC analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.
(3 Reviews)
(6 Publications)
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
ARMC8 RNAi
H00025852-R02
ARMC8 RNAi
Species: Hu
Applications: RNAi, RNAi SP

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Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
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Western Blot: ARMC8 Antibody [NBP1-85149] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-47
ARMC8 Antibody
NBP1-85149
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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Bioinformatics

Gene Symbol ARMC8