ARID2 Antibody

Images

 
Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody used for WB at 0.04 ug/ml. ...read more
Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB
Immunoprecipitation: ARID2 Antibody [NBP1-26615] - Mouse embryonic fibroblasts. Image submitted by a verified customer review.
Immunoprecipitation: ARID2 Antibody [NBP1-26615] - Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ARID2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1785 and 1835 of human AT rich interactive domain 2 using the numbering given in entry NP_689854.2 (GeneID 196528).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARID2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 5-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ARID2 antibody validated for IP from a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-26615 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARID2 Antibody

  • ARID domain-containing protein 2
  • AT rich interactive domain 2 (ARID, RFX-like)
  • BAF200KIAA1557AT-rich interactive domain-containing protein 2
  • BRG1-associated factor 200
  • DKFZp686G052
  • DKFZp779P0222
  • FLJ30619
  • p200
  • Zinc finger protein with activation potential
  • zipzap
  • zipzap/p200

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID2 (AT-rich interactive domain-containing protein 2) is also known as BAF200 and was purified as a factor that associated with PBAF (hSWI/SNF-B). ARID2 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. In addition to the ARID domain, ARID2 also contains a C2H2-type zinc finger and has been implicated as a transcriptional co-activator for serum response factor for the regulation of cardiac genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARID2 Antibody (NBP1-26615) (0)

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Review for ARID2 Antibody (NBP1-26615) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-26615:
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Images Ratings Applications Species Date Details
Immunoprecipitation ARID2 NBP1-26615
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5
reviewed by:
Verified Customer
Immunoprecipitation Mouse 06/13/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedMouse embryonic fibroblasts
SpeciesMouse
LotA-1

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Secondary Antibodies

 

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Verified Customer
06/13/2014
Application: Immunoprecipitation
Species: Mouse

Bioinformatics

Gene Symbol ARID2
Entrez
Uniprot