Arg-Vasopressin Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.0 mg/ml

Order Details

Arg-Vasopressin Antibody Summary

Immunogen
Synthetic arginine-vasopressin conjugated to bovine thyroglobulin.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AVP
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:2000 - 1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Broad species cross-reactivity expected from sequence.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
5.0 mg/ml
Purity
Protein G purified

Notes

Hypothalamus and posterior lobe of the pituitary gland are reliable positive control tissues for IHC experiments.

Background

Vasopressin, also known as arginine vasopressin (AVP), or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Arg-Vasopressin Antibody (NB100-65214). (Showing 1 - 1 of 1 FAQ).

  1. We ordered the Arg-vasopressin Antibody (NB100-65214) (0.05 ml) last week and also received it. I was wondering whether you have any idea about the working dilution since there is nothing indicated in the datasheet?
    • The recommended starting dilution for IHC is 1:100-1:200 and for IF we recommend 1:50-1:250. These are just starting dilutions and should be optimized.

Secondary Antibodies

 

Isotype Controls

Additional Arg-Vasopressin Products

Arg-Vasopressin NB100-65214

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Bioinformatics

Gene Symbol AVP
Entrez
Uniprot