ARFGAP1 Antibody (OTI1F9)


Western Blot: ARFGAP1 Antibody (1F9) [NBP2-00512] Analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ARFGAP1 Antibody (1F9) [NBP2-00512] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1.
Flow Cytometry: ARFGAP1 Antibody (1F9) [NBP2-00512] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARFGAP1 antibody, and then more
Western Blot: ARFGAP1 Antibody (1F9) [NBP2-00512] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell more
Flow Cytometry: ARFGAP1 Antibody (1F9) [NBP2-00512] - Analysis of Hela cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ARFGAP1 Antibody (1F9) [NBP2-00512] - Analysis of Jurkat cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

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ARFGAP1 Antibody (OTI1F9) Summary

Full length recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cells.
Immunogen affinity purified
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  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
Theoretical MW
45.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Monkey

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for ARFGAP1 Antibody (OTI1F9)

  • ADP-ribosylation factor 1 GTPase activating protein
  • ADP-ribosylation factor 1 GTPase-activating protein
  • ADP-ribosylation factor GTPase activating protein 1
  • ADP-ribosylation factor GTPase-activating protein 1
  • ARF GAP 1
  • ARF1 GAP
  • ARF1-directed GTPase-activating protein
  • bA261N11.3
  • FLJ10767
  • GAP protein
  • HRIHFB2281
  • MGC39924


The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol ARFGAP1