APRIN Antibody

Product Discontinued

APRIN Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1200 and 1250 of human Precocious Dissociation of Sisters-5B using the numbering given in entry CAB69911.1 (GeneID 23047).
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDS5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APRIN Antibody

• Androgen-induced proliferation inhibitorKIAA0979androgen induced inhibitor of proliferation
• Androgen-induced prostate proliferative shutoff-associated protein AS3
• androgen-induced shutoff 3
• AS3APRIN
• CG008
• FLJ23236
• PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• sister chromatid cohesion protein PDS5 homolog B

Background

Pds5B is a cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. It plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APRIN Antibody (NB100-754)(1)

Publication using NB100-754

• Hegemann B, Hutchins JR, Hudecz O et al. Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 2011-11-01 [PMID: 22067460]

Bioinformatics

• Gene Symbol: PDS5B
• Entrez:
  • 23047(Human)
  • 100710(Mouse)
• Uniprot:
  • Q9NTI5(Human)