Apolipoprotein E2 Antibody

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Western Blot: ApoE Antibody [NBP1-45355] - Western blot analysis of ApoE2 using recombinant human ApoE2 Lane 1: 50ng Lane 2: 250ng Lane 3: 1ug.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Apolipoprotein E2 Antibody Summary

Immunogen
E. coli expressed recombinant human ApoE2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOE
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant human ApoE2 can be used as a positive control.
Theoretical MW
34.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein E2 Antibody

  • AD2
  • Apo-E
  • apolipoprotein E
  • LDLCQ5
  • LPG

Background

The apolipoproteins are family of amphipathic lipoproteins that plays a crucial role in lipid metabolism. In particular, apolipoprotein E (apo E) is a major component of high density lipoproteins (HDL) chylomicrons and very low-density lipoprotein (VLDL), which functions to remove excess cholesterol. Apo E mediates lipid metabolism and cholesterol homeostasis by binding to several hepatic lipoprotein receptors, such as low density lipoprotein receptor (LDLR) and the LDLR-related protein (1). Apo E deficiency is linked to hyperlipoproteinemia type III; a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease (2). Three major isoforms of Apo E (E2, E3, E4) has been identified, with E3 being the most common in human. E4 presence has been associated to increase probability of developing late-onset form of Alzheimer disease (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol APOE
Entrez
Uniprot