Apolipoprotein B/ApoB Antibody (2E3)



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Apolipoprotein B/ApoB Antibody (2E3) Summary

The antibody reacts with low density lipoproteins of human plasma.
The antibody reacts with low density lipoproteins of human plasma. It is specific for fragment T2, the carboxyterminal thrombolytic peptide of human apo-B. No crossreactions with other human plasma proteins were detected.
IgM Kappa
Sodium sulfate precipitation
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  • ELISA 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10
Application Notes
The antibody can be used for LDL depletion of human plasma, in combination with other antibody for detection of LDL in plasma and for the specific detection of apo-B. The antibody is also useful for detection of apo-B in tissue sections.The antibody can be used in ELISA techniques and in immunohistology on frozen sections. For depletion experiments the antibody should be used in a solid phase system.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C. Do not freeze.
1 ml purified solution with 0.5 mg/ml antibody, 10mM Sodium Phosphate (pH 8.0), 0.1M NaCl and 0.1% (w/v) Mannitol
1mM Sodium Azide
Sodium sulfate precipitation
Reconstitution Instructions
Reconstitute with 1 ml sterilized water and with preservative if preferred.

Alternate Names for Apolipoprotein B/ApoB Antibody (2E3)

  • Apo B-100
  • APOB
  • apoB-100
  • apoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100


The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis (1). Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits (2). It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) was shown to be mediated by LDL receptors on cultured cells (3).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol APOB