Apolipoprotein A-IV/ApoA4 Antibody

Images

 
Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [H00000337-B01P] - Analysis of APOA4 expression in transfected 293T cell line by APOA4 polyclonal antibody. Lane 1: APOA4 transfected lysate(43.56 KDa). Lane 2: ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [H00000337-B01P] - Analysis of purified antibody to APOA4 on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Apolipoprotein A-IV/ApoA4 Antibody Summary

Immunogen
APOA4 (AAH74764.1, 1 a.a. - 396 a.a.) full-length human protein. MFLKAVVLTLALVAVAGARAEVSADQVATVMWDYFSQLSNNAKEAVEHLQKSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKEEIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQAEQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES
Specificity
APOA4 - apolipoprotein A-IV,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
APOA4
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactivity against transfected lysate for WB. It has been used for IHC-P and ELISA.
Publications
Read Publication using H00000337-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-IV/ApoA4 Antibody (H00000337-B01P)(1)

Reviews for Apolipoprotein A-IV/ApoA4 Antibody (H00000337-B01P) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (H00000337-B01P). (Showing 1 - 2 of 2 FAQ).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.
  2. Would this antibody work with Mouse or Rat? And has it been tested?
    • This antibody has never been tested in Mouse or Rat. We will list any applications or species that have been tested and confirmed not to work by customers or ourselves with a (-) sign next to them. If the species or application is simply not present it means we have yet to test it. Testing is typically performed against the human protein. Here is the immunogen used for this antibody against the human sequence: MFLKAVVLTLALVAVAGARAEVSADQVATVMWDYFSQLSNNAKEAVEHLQKSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKEEIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQAEQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES. We do not epitope map this antibody, so it is uncertain where the polyclonal binds along this sequence, and if it will detect mouse or rat samples. I suggest running a blast against the protein to see how high the homology is. Typically our lab doesn't recommend testing anything unless it is at least 85% homologous. If homology is 85% or higher we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOA4
Entrez
Uniprot