APE Antibody

Product Discontinued

APE Antibody Summary

• Immunogen: APEX1 (NP_001632, 1 a.a. ~ 318 a.a) full-length human protein.
• Specificity: APEX1 - APEX nuclease (multifunctional DNA repair enzyme) 1,
• Clonality: Polyclonal
• Host: Mouse
• Gene: APEX1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against immunizing recombinant protein, transfected lysate and tissue lysate for Western Blot. Has also been used for immunofluoresence, and immunohistochemistry (paraffin sections), and ELISA.
• Antagonist:
  • APE1 Redox Inhibitor NBP1-49581

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: This is unpurified antiserum so it is not possible to supply the specific antibody concentration.
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for APE Antibody

• AP endonuclease 1
• APE
• APE1
• APE-1
• APEAPEX nuclease (multifunctional DNA repair enzyme)
• APENAP endonuclease class I
• APEX deoxyribonuclease (apurinic or apyrimidinic)
• APEX nuclease (multifunctional DNA repair enzyme) 1
• APEX nuclease
• APEX1
• Apurinic-apyrimidinic endonuclease 1
• APXAP lyase
• DNA-(apurinic or apyrimidinic site) lyase
• EC 3.1
• EC 4.2.99.18
• HAP1apurinic/apyrimidinic (abasic) endonuclease
• multifunctional DNA repair enzyme
• protein REF-1
• redox factor 1
• Redox factor-1
• REF1 apurinic/apyrimidinic exonuclease
• REF-1

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APEX1
• Entrez:
  • 328(Human)
• Uniprot:
  • NP_001632(Human)