APAF-1 Antibody

Images

 
Western Blot: APAF-1 Antibody [NB100-91661] - Analysis of APAF-1-ALT (H324) pAb in extracts from COLO205 cells.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NB100-91661] - Analyzes of Apaf-1-ALT (H324) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using ...read more
Western Blot: APAF-1 Antibody [NB100-91661] - Extracts from COLO205 cells.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NB100-91661] - Paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related APAF-1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91661
    • Availability
      Product Discontinued

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APAF-1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 301-350 of Human Apaf-1-ALT.
Clonality
Polyclonal
Host
Rabbit
Gene
APAF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of APAF-1-ALT (H324) pAb in extracts from COLO205 cells.
Immunohistochemistry (IHC) analyzes of APAF-1-ALT (H324) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APAF-1 Antibody

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Background

Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein Apaf-1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. Apaf-1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional APAF-1 Products

Bioinformatics Tool for APAF-1 Antibody (NB100-91661)

Discover related pathways, diseases and genes to APAF-1 Antibody (NB100-91661). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for APAF-1 Antibody (NB100-91661)

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Pathways for APAF-1 Antibody (NB100-91661)

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PTMs for APAF-1 Antibody (NB100-91661)

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Research Areas for APAF-1 Antibody (NB100-91661)

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Blogs on APAF-1.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Bioinformatics

Gene Symbol APAF1
Entrez