Antibody Concentration and Clean Up Antibody Purification Kit

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Summary

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Antibody Concentration and Clean Up Antibody Purification Kit Summary

Description
Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores.

FeaturesBenefits
Quickly and easily concentrate antibodies and proteinsEasy and quick to use
Remove non-protein additives such as sodium azide, tris and glycineHigh recoveries, around 60 - 100%
Perform a simple buffer exchange for more favourable conjugation conditionsSuitable for Lightning-Link kits and for other kits


The antibody concentration and clean-up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution.

Kit Type
Antibody Purification Kit

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 4
using
861-0010 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 3 spin cartidges / collecting tube assemblies
  2. 1 bottle of AbSelect Conjugation buffer

Notes

This product is manufactured by Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Review for Antibody Concentration and Clean Up Kit (861-0010) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using 861-0010:
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Flow Cytometry Antibody Concentration and Clean Up 861-0010
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reviewed by:
Anonymous
Flow Mouse 05/15/2014
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Summary

ApplicationFlow Cytometry
Sample TestedAscites fluid from mouse (1 ml)
SpeciesMouse
Lot6518
CommentsFor researchers who produce their own ascites antibodies this kit would probably be very useful since it can purify large amounts of ascites. For researchers who are looking to purify purchased ascites antibodies at a few hundred ul it is not optimal as it would be very expensive. Using 1ml ascites the antibody came out in the 3rd elute at 0.28mg/ml, I would therefore need to concentrate it using the concentration kit to ~1mg/ml before I could use the Lightning-link kits to conjugate it. The kit is very easy to use, although it does take its time with the drip columns. I give it 4 stars because it does what it says on the box, but a cheaper and quicker miniature version would better fit my needs.

Blocking

Blocking DetailsN/A

Primary Anitbody

Dilution RatioN/A

Details

Detection NotesOD at 280nm (1 mg/ml = 1.4 OD, 0.5 mg/ml = 0.7 OD etc) measured by Jenway 7315 spectrophotometre (blank = water).
Fixation DetailsAfter dilution with wash buffer the sample was mixed with 0.5 ml binding buffer and then with the protein G resin (whole vial).
Permeabilization DetailsN/A
Wash DescriptionWash buffer, ~10 min to drop through/wash, 3 washes of column contents once sample/binding buffer/resin mix has dripped through.

Comments

CommentsFor researchers who produce their own ascites antibodies this kit would probably be very useful since it can purify large amounts of ascites. For researchers who are looking to purify purchased ascites antibodies at a few hundred ul it is not optimal as it would be very expensive. Using 1ml ascites the antibody came out in the 3rd elute at 0.28mg/ml, I would therefore need to concentrate it using the concentration kit to ~1mg/ml before I could use the Lightning-link kits to conjugate it. The kit is very easy to use, although it does take its time with the drip columns. I give it 4 stars because it does what it says on the box, but a cheaper and quicker miniature version would better fit my needs.

FAQs for Antibody Concentration and Clean Up Kit (861-0010). (Showing 1 - 1 of 1 FAQ).

  1. Is the NB500-169 still available in the concentration of 2mg/ml? We ordered five vials in the past month with the unit size 0.1 mg. Three of them arrived with a concentration of 2mg/ml, the last two vial with a concentration of 1mg/ml. Can we influence the concentration when ordering?
    • In terms of the concentration of the vials, unfortunately sometimes the concentrations of our products may change. (The concentration of each vial is that stated on the label.) I am sorry, but it is not possible for you to order specifically 2 mg/ml vials of NB500-169. (If you require a concentration of 2 mg/ml, you may be interested in our Antibody Concentration and Clean Up Kit (product code for 1 spin column is 861-0005; 3 spin columns is 861-0010), which you could use to reduce the volume of the antibody by half and hence double the concentration.)

Additional Antibody Concentration and Clean Up Products

Antibody Concentration and Clean Up 861-0010

Blogs on Antibody Concentration and Clean Up.

Easy Antibody Labeling, Regardless of Buffer or Concentration!
Novus Biologicals is excited to announce the release of a new line of Antibody Purification Kits, for use with our popular Lightning Link Antibody Labeling Kits. These new kits allow researchers to label any antibody, regardless of the buffer or conce...  Read full blog post.

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Anonymous
05/15/2014
Application: Flow
Species: Mouse