ANKRD28 Antibody

Images

 
Independent Antibodies: Western Blot: ANKRD28 Antibody [NB100-41419] - Whole cell lysate from HeLa cells (5, 15 and 50 mcg for WB; 1 mg for IP, 20 percent of IP loaded) and 293T (T; 50 mcg) cells. NB100-41419 ...read more
Immunohistochemistry: ANKRD28 Antibody [NB100-41419] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB ...read more
Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419] - FFPE section of human stomach carcinoma. Antibody used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

ANKRD28 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 975 and 1025 of human ankyrin repeat domain 28 (phosphatase interactor targeting K protein) using the numbering given in entry NP_056014.2 (GeneID 23243).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANKRD28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ANKRD28 Antibody

  • ankyrin repeat domain 28
  • Ankyrin repeat domain-containing protein 28
  • KIAA0379PITKPP6-ARS-Aphosphatase interactor targeting K protein
  • Phosphatase interactor targeting protein hnRNP K
  • protein phosphatase 6 ankyrin repeat subunit A
  • serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
  • Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A

Background

PITK was previously identified as a novel PP1 targeting subunit implicated in modulating the phosphorylation of the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K). Through the phosphorylation of PITK at S1013 and S1017 (residues that flank or reside within a PP1C-binding motif), the binding of the PP1 catalytic subunit to PITK, and subsequently the activity of the holoenzyme, are discretely controlled.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Array NB100-41419

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Bioinformatics

Gene Symbol ANKRD28
Entrez
Uniprot