Angiotensin I Antibody Summary
The antibody reacts with the Angiotensin I antigen.
Human Angiotensin (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) synthesed and purified to 98% is linked to bovine serum albumin by means of carbodiimide in random orientation. This conjugate is used to immunize rabbit. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized human albumin and immmunoglobuline fractions in order to remove non-specific antibodies. Specificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II.
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Recommended for quantitave immunoassays of angiotensin I.The antiserum should be diluted for binding starting from 1:100. This antibody is not intended for staining tissue.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
1 ml whole Rabbit antisera
Reconstitute with 1 ml sterilized water and with preservative if preferred.
Alternate Names for Angiotensin I Antibody
- alpha-1 antiproteinase, antitrypsin
- angiotensin II
- angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
- serine (or cysteine) proteinase inhibitor
- Serpin A8
- SERPINA8angiotensin I
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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