Angiotensin I Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-50142
    • Availability
      Product Discontinued

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Angiotensin I Antibody Summary

Immunogen
The antibody reacts with the Angiotensin I antigen.
Specificity
Human Angiotensin (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) synthesed and purified to 98% is linked to bovine serum albumin by means of carbodiimide in random orientation. This conjugate is used to immunize rabbit. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized human albumin and immmunoglobuline fractions in order to remove non-specific antibodies. Specificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II.
Clonality
Polyclonal
Host
Rabbit
Gene
AGT
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
Application Notes
Recommended for quantitave immunoassays of angiotensin I.The antiserum should be diluted for binding starting from 1:100. This antibody is not intended for staining tissue.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1 ml whole Rabbit antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 1 ml sterilized water and with preservative if preferred.

Alternate Names for Angiotensin I Antibody

  • alpha-1 antiproteinase, antitrypsin
  • angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • angiotensinogen
  • ANHU
  • FLJ92595
  • FLJ97926
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional Angiotensin I Products

Angiotensin I NBP1-50142

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Bioinformatics

Gene Symbol AGT