Angiopoietin-1 Antibody

Product Discontinued

Angiopoietin-1 Antibody Summary

• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Specificity: This antiserum is directed against mouse angiopoietin 1 and shows no reactivity with mouse angiopoietin 2. A 57 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin 1. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPT1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Immunohistochemistry 1:200-1:800
  • Immunohistochemistry-Paraffin 1:200-1:800
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:2000
• Application Notes: Suitable for western blotting, Immunohistochemistry and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background Due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close - their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.

Reactivity Notes

Partially cross reactivity is noted with human angiopoietin.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Unpurified

Alternate Names for Angiopoietin-1 Antibody

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Anti Antiopoietin-1 Antibody recognizes Angiopoietin-1 (Ang-1) that has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-1 Antibody (NB600-629)(3)

Publications using NB600-629

• Yin GN, Jin HR, Choi MJ et al. Pericyte-Derived Dickkopf2 Regenerates Damaged Penile Neurovasculature Through an Angiopoietin-1-Tie2 Pathway. Diabetes. 2018-03-20 [PMID: 29559443] (WB, Mouse)
• Yin GN, Kim WJ, Jin HR et al. Nerve Injury-Induced Protein 1 (Ninjurin-1) Is a Novel Therapeutic Target for Cavernous Nerve Injury-Induced Erectile Dysfunction in Mice. J Sex Med 2013-04-02 [PMID: 23551591]
• Chade, A R, Krier, J D, Textor, S C, Lerman, A, Lerman, L O. Endothelin-A Receptor Blockade Improves Renal Microvascular Architecture Function in Experimental Hypercholesterolemia, . 17, 3394-3403. 2006-01-01 [PMID: 17082239]

Bioinformatics

• Gene Symbol: ANGPT1