ANAPC2 Antibody (OTI1H8)


Western Blot: ANAPC2 Antibody (1H8) [NBP2-01124] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ANAPC2 Antibody (1H8) [NBP2-01124] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2.
Flow Cytometry: ANAPC2 Antibody (1H8) [NBP2-01124] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANAPC2 antibody, and then more
Western Blot: ANAPC2 Antibody (1H8) [NBP2-01124] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates more

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ANAPC2 Antibody (OTI1H8) Summary

Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.
Immunogen affinity purified
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  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human, Rat, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for ANAPC2 Antibody (OTI1H8)

  • anaphase promoting complex subunit 2
  • anaphase-promoting complex subunit 2
  • APC2KIAA1406Cyclosome subunit 2
  • RP11-350O14.5


A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Gene Symbol ANAPC2