Aminoacylase/ACY1 Antibody (1D4)

Images

 
Western Blot: Aminoacylase 1 Antibody (1D4) [NBP2-00970] Analysis of extracts (35ug) from 9 different cell lines by using anti-Aminoacylase 1 monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - Staining of paraffin-embedded Human prostate tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Flow Cytometry: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Aminoacylase 1 antibody, ...read more
Western Blot: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aminoacylase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunohistochemistry-Paraffin: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - Staining of paraffin-embedded Human Kidney tissue using anti-Aminoacylase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aminoacylase 1 Antibody (1D4) [NBP2-00970] - Staining of paraffin-embedded Human liver tissue using anti-Aminoacylase 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
1D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Aminoacylase/ACY1 Antibody (1D4) Summary

Immunogen
Full length human recombinant protein of human ACY1(NP_000657) produced in HEK293 cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aminoacylase/ACY1 Antibody (1D4)

  • ACY1
  • ACY-1
  • ACY1D
  • acylase
  • aminoacylase 1
  • Aminoacylase
  • aminoacylase-1
  • EC 3.5.1
  • EC 3.5.1.14
  • N-acyl-L-amino-acid amidohydrolase

Background

This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACY1
Entrez